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Objective:To evaluate the effects of sleep fragmentation on vascular cognitive impairment (VCI) and the relationship with central inflammation and oxidative stress in rats.Methods:Forty-eight male Sprague-Dawley rats, aged 8-10 weeks, weighing 210-240 g, were divided into 4 groups ( n=12 each) by a random number table method: sham operation group (group Sham), sham operation + sleep fragmentation group (group Sham+ SF), group VCI and VCI+ sleep fragmentation group (group VCI+ SF). VCI was induced by ligating bilateral common carotid arteries of anesthetized rats.In VCI+ SF and Sham+ SF groups, the sleep fragmentation model was established starting from day 26 after inducing VCI, and the contextual fear conditioning test and open field test were performed on day 29.After the end of the contextual fear conditioning test, the contents of tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-6) were determined by enzyme-linked immunosorbent assay, the hippocampal superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), malondialdehyde (MDA) and iron contents were measured using microplate method, and the expression of nuclear factor-kappa B (NF-κB), caspase-3 and glutathione peroxidase 4 (GPX4) was determined using Western blot. Results:Compared with group Sham, the number of crossing lattices and standing on back legs and percentage of time spent freezing were significantly decreased, the contents of SOD and GSH-Px were decreased, the contents of MDA, IL-6, TNF-α and iron were increased, the expression of NF-κB and caspase-3 was up-regulated, and the expression of GPX4 was down-regulated in group VCI ( P<0.05). Compared with Sham+ SF and VCI groups, the number of crossing lattices and standing on back legs and percentage of time spent freezing were significantly decreased, the contents of SOD and GSH-Px were decreased, the contents of MDA, IL-6, TNF-α and iron were increased, the expression of NF-κB and caspase-3 was up-regulated, and the expression of GPX4 was down-regulated in group VCI+ SF ( P<0.05). Conclusion:Sleep fragmentation can aggravate VCI, and the mechanism may be related to the induction of central inflammation and oxidative stress, which leads to increased apoptosis and ferroptosis in hippocampal neurons of rats.
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Clinical data of 349 patients with postmenopausal ovarian cystic and cystic solid tumors undergoing surgical treatment in Beijing Obstetrics and Gynecology Hospital affiliated to Capital Medical University from May 2013 to May 2017 were retrospectively analyzed.Postoperative pathology confirmed 100 cases of malignant tumor,228 cases of benign tumor,and 21 cases of borderline tumor.The malignant risk index (RMI) score was calculated based on the preoperative pelvic ultrasound and serum CA125 level,and the score＜200 was classified as benign tumors and ≥200 as malignant tumors.The postoperative pathological results were used as gold standard,the sensitivity,specificity,accurate rate of RMI in diagnosis of malignant tumors was 0.840(84/100),0.987 (225/228) and 94.2％ (309/328),respectively.The consistency of RMI with pathological diagnosis was high (Kappa=0.858,P＜0.05).The study suggests that RMI has a high degree of credibility for the evaluation of postmenopausal ovarian cystic and cystic solid tumors.
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Objective To explore the efficacy of hysteroscopy combined with laparoscopy in the diagnosis and treatment of Herlyn-Werner-Wunderlich syndrome (HWWS).Methods The clinical data of 23 patients with HWWS from January 2009 to December 2015 treated with combination of laparoscopy and hysteroscopy were retrospectively analyzed.The patients had different degrees of dysmenorrhea (17 cases),abnormal vaginal discharge (17 cases),and infertility (4 cases) before surgery.During followup,we observed the symptom and pregnancy outcomes after surgery.Results The patients were followed up for 3-36 months (average,24 months).The vaginal irregular bleeding and abnormal secretions disappeared in 17 patients;dysmenorrhea relieved partly in 17 cases;4 patients who had fertility requirements gave birth to babies.Conclusion Combination of hysteroscopy and laparoscopy sufficiently clears genital malformation of HWWS and solves the lower genital tract obstruction.
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Objective To establish a method for determining the dissolution of isoniazid tablet in vitro and evaluate the dissolution profiles.Methods The paddle method was used for the dissolution test and the rotation rate was set at 50 r/min.The hydrochloric acid solution (pH 1.2),acetate buffer solution (pH 4.5),phosphate buffer solution (pH 6.8) and water (900 mL) were used as the dissolution media.HPLC was used for the determination of dissolution quantity.Results There was a good linear relationship between the quality concentration of i soniazid and peak area in the range of 0.1981-0.9904μg (r =0.9993).The average recovery was 100.2％.Precision,reproducibility,and specificity tests were good.Among the determination of 16 manufactures,the dissolution profiles in water of four manufactures were not similar with Sandoz reference preparation.Conclusion The HPLC method is simple.The accuracy and specificity of determination of isoniazid dissolution are improved.There is significant difference in the dissolution profiles between different manufactures.The method can be used for the determination of dissolution curves for isoniazid tablets.
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ABSTRACT:Objective To analyze the effects of antisurvivin oligonucleotide (Survivin ASODN)on IL-6/STAT3 signaling pathway and down-stream cancer genes of ovarian cancer SKOV3 cell line and to detect the changes of invasive ability of cell line in order to explore the role of Survivin ASODN in reducing metastasis and recurrence of ovarian cancer and its potential clinical value.Methods ASODN was transfected into SKOV3 cells by lipofectamineTM2000 in ASODN group,and lipofectamineTM 2000 was introduced in control group.The invasive ability in ASODN and control groups was detected by Transwell chamber.The expressions of interieukin-6 (IL-6 ), signal transducer and activator of transcription 3 (STAT3 ), Survivin, and vascular endothelial growth factor (VEGF)in ovarian cancer cell line at mRNA level were detected by Real-time PCR.The expressions of IL-6 , STAT3 ,signal transducer and activator of transcription 3 phosphorylation (p-STAT3 ),Survivin,and VEGF-A in ovarian cancer cell line at protein level were detected by Western blot.Results The expressions of IL-6,STAT3, Survivin,and VEGF in ovarian cancer cell line at mRNA level were significantly down-regulated in ASODN group (P<0 .0 5 ).IL-6 ,STAT 3 ,p-STAT 3 ,Survivin and VEGF-A protein levels in ovarian cancer cell line were significantly down-regulated in ASODN group (P<0.05).The invasive ability was significantly reduced by ASODN (P<0.01).Conclusion ASODN targeting Survivin could reduce the invasive ability of ovarian cancer cell line. The mechanism may be blocking IL-6/STAT3 signaling pathway and its down-stream cancer genes of ovarian cancer cell line.ASODN targeting Survivin may have clinical value in preventing and treating the metastasis and recurrence of ovarian cancer.
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Total 215 patients with simple varicosis of great saphenous vein underweni surgical treatment in our hospital from February 2007 to January 2012,including 75 cases receiving traditional high ligation segmentation stripping operation (group A),69 cases receiving endovenous laser light therapy (group B),and 71 receiving minimally invasive translucent atherectomy (group C).The efficacy and postoperative complications were compared among 3 groups.The operation time,intraoperational bleeding and length of hospitalization in groups B and C were significantly less than those in group A (P ＜ 0.05).The hospitalization cost of groups A and B was significantly lower than that of group C (P ＜ 0.05).The incidence of subcutaneous induration in groups A and B was significantly higher than that of group C (P ＜ 0.05).The incidence of subcutaneous congestion in group A was significantly higher than that in groups B and C (P ＜0.05).The recurrence rate during 2-years of follow-up in groups A and B was higher than that in group C (x2 =8.592,5.672,P =0.003,0.016).Three surgical modalities in treatment of great saphenons varicose veins have advantage and disadvantages,the appropriate methods should be chosen based on the characteristics of procedures and the condition and intention of patients.
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[Summary] There are a variety of treatment methods for stress urinary incontinence ( SUI) .Surgery is effective but has high risks, only to adapt to the limited population .Conservative treatment has a high recurrence rate and poor long-term results.Local injection of stem cell has been proposed as a new minimally invasive therapy of SUI .Stem cells, with the ability to transform into muscle cell , secrete various growth factors , promote local nerve and muscle tissue regeneration , and repair sphincter dysfunction .This article reviewed on therapy of skeletal muscle-derived stem cells and the associated risks of treatment .
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Objective To develop a optimized rehabilitation protocol for acupuncture, Tuina and ultrasonic treatments of upper limb motor dysfunction after cerebral infarction.Methods An orthogonal design with three factors (acupuncture as factor A, Tuina as factor B and ultrasound as factor C) and two levels was used. Eighty cerebral infarction patients with upper limb motor dysfunction were randomly allocated to eight groups, 10 cases each. The National Institute of Health Stroke Scale (NIHSS) score and the simplified Fugl-Meyer Assessment (FMA) score for upper limb motor function were recorded after four weeks of treatment. Results There were no statistically significant pre-treatment differences in the NIHSS score and the FMA score for the upper limb between the groups (P>0.05). For an improvement in NIHSS, factors A and B were both significant (bothP0.05). Factors A, B and C were all significant for an improvement in the FMA score for the upper limb (P<0.05,P<0.01,P<0.05). A2B1C1 was the optimal protocol.Conclusions A combination of bilateral acupuncture, Tuina and ultrasound is an optimized protocol for treating upper limb motor dysfunction after cerebral infarction. It can effectively reduce upper limb motor dysfunction.
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<p><b>OBJECTIVE</b>To explore the most appropriate imaging examinations for solitary osteochondroma in primary hospitals.</p><p><b>METHODS</b>A retrospective evaluation was performed to analyze the imaging examinations (including X-ray, CT, and ultrasound) of 62 patients undergoing surgeries for solitary osteochondroma in terms of the diagnostic results and preoperative expenditure of the patients.</p><p><b>RESULTS</b>X-ray and CT have a high diagnostic value for solitary osteochondroma, but X-ray cannot display cartilage and lesions in surrounding soft tissues. Ultrasound is limited in diagnosis of solitary osteochondroma, but performed well as CT in displaying cartilage and lesions in surrounding soft tissues. There was no statistical difference between combined X-ray and ultrasound and CT in diagnosis of solitary osteochondroma (P>0.05), but the former had a lower cost (P<0.05).</p><p><b>CONCLUSION</b>X-ray combined with ultrasound has almost comparable value with CT in the diagnosis of solitary osteochondroma and is more practical for application in primary hospitals.</p>
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Adolescent , Adult , Child , Female , Humans , Male , Young Adult , Bone Neoplasms , Diagnostic Imaging , Osteochondroma , Diagnostic Imaging , Retrospective Studies , Tomography, X-Ray Computed , Ultrasonography , X-RaysABSTRACT
Good self-management behaviors can control symptoms of the patients with osteoarthritis, improve the patients' joint function and quality of life. Patients' self-management behaviors have been impacted by disease knowledge, self-effcacy, emotional state, and social support. All the above factors should been taken into full consideration when intervening. Self-management program is an intervention mode which can improve patient self-management behaviors and promote patient health.
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Objective To investigate the association of prevalence of periodontitis with metabolic syndrome (MS). Methods Data were analyzed from 1 650 Uygur rural residents in Moyu County. The subjects, aged over 18 years, were sampled randomly from 15 villages out of total 364 villages. Questionnaire, oral examination, and blood biochemical indicators were collected. The subjects were divided into groups with and without periodontitis based on chronic periodontitis diagnostic criteria, and the group with periodontitis was further divided into subgroups, each with mild, moderate, and severe periodontitis respectively. The diagnosis of MS was madeaccording to the definition of the International Diabetes Federation in 2005. Results Among 1 415 subjects whosedata were complete, there were 275 ( 19.4% ) subjects with MS and 934 (66.0%) subjects with periodontitis. The prevalence of MS was higher in the group with periodontitis than that without perionontitis (23.1% vs 12.3%, x2=23.9, P<0. 001 ). The prevalence of MS was increased with the grade of periodontitis, being 19.8%, 20.8%,27.6% in the mild, moderate, and severe periodontitis groups, respectively(x2= 31.9, P<0. 001 ). Multiple logistic regression analysis showed that the risk of MS increased with the grade of periodontitis, with OR 1. 6, 1.7,1.9, respectively, in the groups with mild, moderate, and severe periodontitis compared with that without perionontitis ( P<0.05 or P<0.01 ). Conclusions The prevalence of MS was related to periodontitis in the Uygur nationality and increased with the grade of periodontitis.
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0.05). The morphological changes were observed in the cochlea. Moreover, the changes of cochlear tissues under the optical microscope wasa lot of akaryotes were found in the blood capillary of cartilaginous tissue in the rats of 30 minutes after rammed; a great quantity inflammatory cells were observed in the blood capillary of cartilaginous tissue in the rats of 1 day rammed. The changes of morphology coincided with the results of DPOAE. CONCLUSION Outer hair cells may be injured by cerebral concussion and the changes were showed by DPOAE and ABR.
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OBJECTIVE To analyze the cleaning efficiency of using Ruhof multi-enzyme detergent to clean endoscopies. METHODS Take 80 sets of used endoscopies as samples and clean them with multi-enzyme detergent in "four-trough" way as instructed by the Ministry of Health.After cleaning,the changes in bio-burden residual on the endoscope′s surface and jet obstruction were observed. RESULTS After cleaning the endoscopes with Ruhof multi-enzyme detergent,the bio-burden residual on the endoscope′s surface as well as jet obstruction,and the surface cleanness evaluation value had decreased enormously.The difference value before and after cleaning was significant(P